| Identifiers | ||
| Product Name | FOXO Reporter Kit (PI3K/AKT Signaling Pathway) | |
| Catalogue No. | CM854656 | |
| Description | The FOXO Reporter kit is designed to monitor activity of the PI3K/AKT signaling pathway and the transcriptional activity of FOXO proteins in cultured cells. The kit contains the transfection-ready FOXO3 expression vector and the FOXO luciferase reporter vector, which is a PI3K/Akt pathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimers of the FOXO responsive element located upstream of a minimal promoter. The FOXO reporter is premixed with a constitutively-expressing Renilla (sea pansy) luciferase vector, which serves as an internal control for the transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as negative control. The non-inducible luciferase vector also contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The negative control is critical to determining pathway-specific effects and background luciferase activity. | |
| Application | • Monitor PI3K/AKT signaling pathway activity and FOXO transcriptional activity. |
|
| Product Type | Reporter Assay Kit | |
| Storage Condition | refer to Technology Column | |
| Brand | Packing | Pricing | Quote request |
| IL | 500 reactions | Inquiry | Ask Quote |
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