| Identifiers | ||
| Product Name | SUV39H2 Assay Kit | |
| Catalogue No. | CM853853 | |
| Description | The SUV39H2 Direct Activity Assay Kit is designed to measure SUV39H2 activity for profiling and screening applications, using purified SUV39H2. The SUV39H2 Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, HRP-labeled antibody against methylated lysine residue of Histone H3, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV39H2 enzymes for 100 enzyme reactions. The key to the SUV39H2 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only two simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, HRP-labeled antibody is added. Finally, HRP substrate is added to produce chemiluminescence that can then be measured using a chemiluminescence reader. COMPONENTS: 4x HMT assay buffer: 3 ml, Blocking buffer: 50 ml, S-adenosylmethionine: 250 µl @ 400 µM; HRP labeled Antibody 1, 100 µl, HRP chemiluminescent substrate (2 components) 6 ml each; SUV39H2, 4 µg; 1 black plate precoated with histone substrate. APPLICATIONS: Great for studying enzyme kinetics and HTS applications. | |
| Product Type | Assay Kit | |
| Storage Condition | Mixed storage +4°C and -80°C | |
| Brand | Packing | Pricing | Quote request |
| IL | 96 reactions | Inquiry | Ask Quote |
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