| Identifiers | ||
| Product Name | DNMT3B Assay kit | |
| Catalogue No. | CM802645 | |
| Description | The DNMT3B Direct Activity Assay Kit is designed to measure DNMT3B activity using purified DNMT3B/3L or cell extracts containing DNMT3B. The DNMT3B Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with DNMT substrate, an antibody against 5-methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT assay buffer, and purified DNMT3B/3L complex for 100 enzyme reactions. The key to the DNMT3B Direct Activity Assay Kit is a highly specific antibody that recognizes 5-methylcytosine of the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT3B activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT3B/3L for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader. COMPONENTS: DNMT3B/3L 20 µg, 400 µM S-adenosylmethionine 500 µl, Anti-5-methylcytosine antibody 25 µl, 4x DNMT assay buffer 2 3 ml, Blocking buffer 50 ml, Secondary HRP-labeled antibody 10 µl, HRP chemiluminescent substrate (2 components) 6 ml each, Black plate pre-coated with DNMT 3B substrate. APPLICATIONS: Useful for studying enzyme kinetics and HTS applications. STABILITY: 1 year when stored as listed. REFERENCES: 1. Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106. | |
| Product Type | Assay Kit | |
| Storage Condition | Mixed storage +4°C and -80°C | |