Identifiers  
Product Name Lysyl Oxidase-like 4 (LOXL4) Human Recombinant Protein
Catalogue No. CM725692
Description Human LOXL4 (GenBank Accession No. NM_032211), amino-acids 500-end, with C-terminal Flag-tag, MW = 31 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Activity: Enzyme activity is assayed by an AmplexRed/Peroxidase coupling method using 1,5-diaminopentane as a substrate. Purified LOXL-4 (0.25-1 ng/ul) is incubated with 3 mM 1,5-diaminopentane, 0.4 mM AmplexRed and 0.2 unit/ul peroxidase in 100 ul of the assay buffer (1.2M Urea, 50 mM borate pH=8.8) for 15 min at 30°C. Fluorescence intensity is measured every 7 minute at 530ex/590em. Fomulated in: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT. Stability: >6 months at –80°C. References: 1. Gorogh T. et al., Int. J. Oncol. 33 (5), 1091-1098 (2008). 2. Kim D.J. et al., Biochem. Biophys. Res. Commun. 373 (4), 521-527 (2008).
MW 31 kDa
Product Type Recombinant Protein
Host Sf9 insect cells
Source Antibody human
Storage Condition >6 months at -80°C
 

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