| Identifiers | ||
| Product Name | Turbo3C Protease | |
| Catalogue No. | CM725659 | |
| Description | Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly. The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C with high specific activity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. This HRV3C Protease is a 47 kDa protein with both GST and His tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag. Specific Activity: The activity of HRV3C Protease is tested using a control target protein. One (1) μg of HRV3C Protease has at least 1 unit activity conventionally used by other suppliers (1 unit of HRV3C protease cleaves >95% of 100 μg of control target protein at 4°C for 16 hours). No non-specific activity has been observed under the same condition with HRV3C Protease to control target protein ratio of 1:10. Prolonged incubation (several days) under the same condition does not show any non-specific cleavage. Formulated In: 25 mM Tris-HCl pH 8.0, 50 mM NaCl, 1 mM TCEP, 50% glycerol. Stability: Store at -20°C. Stable at room temperature for at least two week without loss of any activity. It retains full activity after incubation at 37°C for one week. | |
| MW | 47 kDa | |
| Product Type | Enzyme | |
| Source Antibody | rhinovirus | |
| Storage Condition | -20 | |
| Brand | Packing | Pricing | Quote request |
| IL | 1000 units | Inquiry | Ask Quote |
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