| Identifiers | ||
| Product Name | Urea Broth Base (Without Supplement , see List of Reagents & Supplements Ref. 6-083) | |
| Catalogue No. | CM511225 | |
| Description | According to Rustigian and Stuart, this Urea Broth is excellent for diagnosing enterobacteria, since within this family, only Proteus may alkalinize the medium over pH 8,1. Despite the fact that some authors prefer a buffer of potency 10 or 100 times lower to obtain faster results for saving the time (about 2 hours) does not compensate for the instability of the medium. Urease production is shown by the indicator turning to dark pink, produced by strong alkalinization by ammonium. With plenty of inoculum (2-3 loops in 3-5 mL of medium), Proteus produces the colour change after 6-8 hours, meanwhile other positive enterobacteria need up to 24-48 hours. | |
| Application | Liquid diagnostic medium according to Rustigian and Stuart formulation. | |
| CAS Number | Urea Broth | |