| Identifiers |
 |
| Product Name |
PALCAM Agar Base (Without Supplement , see List of Reagents & Supplements Ref. 6-110CASE) |
| Catalogue No. |
CM510851 |
|
Description |
Palcam Agar is based in the formulation described initially by van Netten et cols., which has a high selectivity and a good colonial differentiation. Selectivity is achieved by the inclusion of lithium chloride, acriflavine, polymixin B and ceftacidine, since they inhibit the growth of almost all the gramnegative bacteria and most of grampositive companion bacteria. Listeria hydrolyze esculin to esculetin, which reacts with ferric ammonium citrate producing a dark precipitate colouring the colonies to green-grey with beige halos. The colonies of enterococci or staphylococci that may overpass the high selectivity of this medium can be easily recognized, since they use mannitol and produce yellow colonies and halos, contrasting with the cherry red colour of medium. However, when there are many Listeria colonies, the entire medium turns dark, and the differentiation can be interfered. In these cases it is advisable to perform a more diluted inoculation. |
|
Application |
Solid, selective and differential medium for the detection, enumeration and isolation of Listeria spp. According ISO standards 11290-1 and 11290-2. |