| Identifiers |
 |
| Product Name |
OxidatiON-FermentatiON Fluid Medium (O/F Medium) |
| Catalogue No. |
CM510849 |
|
Description |
Hugh and Leifson obtained a clear differentiation of gram-negative bacteria with this medium. They classified them into three categories: fermentative, oxidative and inactive. The strain to be studied is inoculated in two long narrow tubes (12x120 mm) by deep stab and one is covered with a vaseline layer to induce an anaerobic environment that forces the strain to carry out the fermentative metabolism. Fermentative organisms give a copious production of acid in both the tubes, and it is indicated by the yellow colouration of the bromothymol blue indicator. The bacteria following the oxidative metabolism carry out this reaction only in the tube without the vaseline butin the other one, which is closed, they change insignificantlly or simply do not grow. Inactive strains do not use sugars and therefore do not induce any change in both the tubes. However, some times there is a slight blue colouration in the open tube, probably due to alkalinization by peptone degradation. Some authors hav |
|
Application |
Fluid medium according to the Hugh and Leifson formulation, for determining the oxidative and/or |