| Identifiers |
 |
| Product Name |
EE Broth |
| Catalogue No. |
CM510292 |
|
Description |
As the name suggests, this medium is for the enrichment of enterobacteria, and is a modification by Mossel(1963) of the classic Brilliant Green Bile 2% Broth (Ref. 02-041). Substitution of lactose by glucose makes it more suitable for enteric bacteria detection, whether gas or non-gas-producer, in food and different samples. Usual recommended technique is as follows : sample to be studied is added to sterile broth at a proportion of 10%. After strong homogenization, the mixture is incubated for a period of18-20 hours at 35-37°C. Afterwards, subcultures are performed on a solid media appropiate for the selective enterobacteria isolation. For this step, Violet Red Bile Agar (Ref. 01-164) is specially recommended, though there are also the MacConkey (Ref. 01-118), Deoxycholate or Brilliant green based media. From the suspected colonies on this media, identification can be performed following the common methodology |
|
Application |
Liquid culture medium for the enrichment of enterobacteria from food samples according to ISO 8523 standard |