| Identifiers |
 |
| Product Name |
DNAse Agar |
| Catalogue No. |
CM510271 |
|
Description |
Jeffries, Holtman and Guse (1957) incorporated DNA into a general medium with agar to study bacterial and fungal DNAse production. Microorganisms that are able to produce DNAse, break DNA, reducing it to nucleotide fragments. This reaction is observed by the appearance of a clear zone surrounding the growth, the rest of the plate remaining turbid. The process is as follows : Hydrochloric acid reacts with DNA producing white precipitates that make the medium turbid, although it does not react with nucleotide fragments. The same authors also observed that there is a correlation between coagulase production and DNase activity, thus DNAse Medium may be used as a laboratory test to diagnose pathogenic staphylococci. Mannitol fermentation may be simultaneously determined if 10 g of mannitol and 0,025 g of phenol red are added to 1 L of DNAse Agar, before sterilization. Positive results in both tests will determine with more certainty that the microorganism is a pathogenic Staphylococcus aure |
|
Application |
Solid culture medium for the determination of the deoxyribonuclease activity of microorganisms, especially of staphylococci and Serratia sp. |