Identifiers |
 |
Product Name |
Annexin V-Cy5 Apoptosis Kit |
Catalogue No. |
CM261749 |
Description |
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NADᶧ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. BioVision’s Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (λ = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples. |
Application |
100 assays. Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. Cy5 shows bright blue fluorescence. The assay is very useful for triple labeling of an apoptotic cell with green and red signals. |
Storage
Condition |
+4°C |