Identifiers |
 |
Product Name |
Annexin V-FITC Apoptosis Kit |
Catalogue No. |
CM261747 |
Description |
Glutathione Reductase (GR, EC 1.8.1.7) catalyzes the NADPH-dependent reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH), which plays an important role in the GSH redox cycle that maintains adequate levels of reduced GSH. A high GSH/GSSG ratio is essential for protection against oxidative stress. BioVision’s Glutathione Reductase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay for measuring GR activity in biological samples. In the assay, GR reduces GSSG to GSH, which reacts with 5, 5′-Dithiobis (2-nitrobenzoic acid) (DTNB) to generate TNB2- (yellow color, λmax = 405 nm). The assay can detect 0.1 – 40 mU/ml GR in various samples. |
Application |
100 assays. Detecting apoptosis in living cells by flow cytometry or fluorescence microscopy. |
Storage
Condition |
+4°C |