| Identifiers |
 |
| Product Name |
T4 DNA Ligase (5 u/ul) |
| Catalogue No. |
CM261722 |
|
Description |
250 u. T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor. One |
|
Application |
250 u. T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor. One unit of T4 DNA ligase is the amount of enzyme activity required to ligate EcoR I digested 1.0 g of plasmid DNA in 5 minutes at 20oC in a 20 l Reaction. |
| MW |
94.0 kDa |
| Source
Antibody |
E. coli |
| Storage
Condition |
-80°C |